An experimental study on effects of active vitamin D on improvement of abnormal calcium homeostasis and bone metabolism induced by high salt diet in animals
Objective To observe the overall changes in the calcium regulatory network after a high salt diet,and ex-plore the effects of supplementing with active vitamin D on calcium homeostasis imbalance and bone metabolism abnormalities caused by a high salt diet.Methods 24 3-month-old male SD rats were randomly divided into control group(Con),high-salt diet group(HN),control+active vitamin D group(Con+VD)and high-salt+active vitamin D group(HN+VD),with 6 rats in each group.After 4 weeks of intervention,the body weight,systolic blood pressure,24 h urine volume,blood and urine electrolytes,kidney function,and the levels of serum angiotensin Ⅱ,aldosterone,atrial natriuretic peptide,parathyroid hor-mone(PTH),fibroblast growth factor 23,25-hydroxyvitamin D,type I procollagen N-terminal pro-peptide(PINP),C-termi-nal cross-linked telopeptide of type Ⅱ collagen(CTX-Ⅱ)were determined and compared.Renal pathology was evaluated by Masson and PAS staining,and the expressions of cytochrome P450 family 27 subfamily B member 1(CYP27B1)and cyto-chrome P450 family 24 subfamily A member 1(CYP24A1)in kidney were analyzed by immunohistochemical staining.Results Compared with the Con group,the HN group had higher levels of urinary calcium/creatinine,serum PTH,PINP and CTX-Ⅱ,with statistical significance(P＜0.05);CYP27B1 expression was down-regulated,CYP24A1 expression was up-reg-ulated,and bone trabeculae decreased in the HN group.Compared with the HN group,the HN+VD group had lower levels of serum PTH,PINP and CTX-Ⅱ,and the differences were statistically significant(P＜0.05);the bone trabeculae increased in the HN+VD group.Conclusion A high-salt diet can inhibit the synthesis of active vitamin D,increase PTH level,affect cal-cium homeostasis and bone metabolism.Supplementation with active vitamin D can ameliorate these effects to some extent.
active vitamin Dhigh-salt dietcalcium homeostasisbone metabolism
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