A study of IL-18R as a potential biomarker for secondary injury of intracerebral hemorrhage
Objective To explore the role of interleukin-18 receptor(IL-18R)in intracerebral hemorrhage(ICH),evaluate its potential as a potential biomarker for ICH,and reveal its underlying mechanism in the neuroinflammation and nerve injury.Methods Wild type C57BL/6 mice were used to establish an ICH model through autologous blood injection.CT ima-ging,nerve function score,grip strength test and foot-fault test were used to evaluate the modeling results.Western blotting was used to detect the expression of IL-18R in brain tissue before and after the modeling.The immunofluorescence co-localiza-tion was used to track the localization of 1L-18R before and after the modeling.Results CT imaging showed the presence of hematomas after modelling.Compared with the-sham surgery group,the nerve function score of the modeling group decreased,tension value in grip strength test decreased,bilateral difference in foot-fault test increased,and expression of IL-18R in-creased.IL-18R co-localized with neurons both before and after modelling.Conclusion IL-18R may play an important role in the secondary neurological impairment after ICH in mice.The expression of IL-18R significantly increases in the ICH model and IL-18R exhibits co-localization neurons,suggesting that IL-18R may be involved in the mechanism of neuroinflammation and has the potential to serve as a biomarker for ICH.Further research on its mechanism may provide new strategies for the treatment of ICH.
万方数据
维普
