山茱萸药材质量评价研究
Study on Quality Evaluation of Cornus Officinalis
郝延军 1胡静 2桑育黎3
作者信息
- 1. 辽宁省食品药品检验所,辽宁沈阳110023
- 2. 沈阳市食品药品检验所,辽宁沈阳110005
- 3. 辽宁大学药学院,辽宁沈阳110036
- 折叠
摘要
目的:比较不同产地山茱萸中马钱苷的含量,考察山茱萸药材3年的稳定性和含果核多少对山茱萸中马钱苷含量的影响.方法:采用高效液相色谱梯度洗脱法对山茱萸中马钱苷进行含量测定.结果:马钱苷进样量在0.077376～4.836μg范围内线性关系良好,平均加样回收率为97.9％,RSD=1.30％.结论:不同产地山茱萸药材中马钱苷含量在0.79％～0.92％范围内,药材中马钱苷含量在3年内稳定性良好.未去核山茱萸的马钱苷含量低于0.60％,其他16批山茱萸药材含马钱苷不少于0.60％.
Abstract
Objective: To compare the contents of loganin in Comus officinalis from different distribution areas;study the quality stability of Comus officinalis for three years; research the effect of fruit stones to the content of loganin in Comus officinalis. Methods: The HPLC method was performed on Diamonsil Clg(4. 6 x250mm,5urn)column by gradient elution with a mixture liquid of acetonitrile and 0.05% (v/v) phosphoric acid in water. The detective wavelength was 240 nm. Results : The calibration curve of loganin was linear over the range of 0. 077376 -4. 836u,g,The average vecovery was 97. 9% with RSD 1. 3%. Conclusion: The content of loganin in Comus officinalis from different distribution areas are among 0. 79% - 0. 92%. The quality stability of Cornus officinalis for three years is good. The contents of loganin in Comus officinalis with fruit stones are less than 0. 60% ;the others are more than 0. 60%.
关键词
山茱萸/马钱苷/梯度洗脱/六味地黄丸Key words
Cornus officinalis/loganin/gradient elution/Liuwei Dihuang Pill引用本文复制引用
出版年
2012
国家科技期刊平台
NSTL
维普
万方数据