摘要
目的:观察健脾消癥方对胃癌细胞株MGC803增殖、凋亡和细胞周期的影响.方法:(1)采用MTT法观察不同浓度的健脾消癥方对胃癌细胞株MGC803增殖的抑制作用；(2)使用Annexin V/PI荧光双染法检测健脾消癥方诱导肿瘤细胞进入凋亡的比例；(3)应用流式细胞仪检测健脾消癥方对MGC803细胞周期的影响.结果:(1)健脾消癥方对MGC803细胞有较强的抑制增殖的作用,并呈一定的浓度依赖性.(2)健脾消癥方高、中、低浓度组对胃癌细胞株MGC803作用24h后,早期凋亡率分别为49.83％、55.29％、16.73％.健脾消癥方高、中、低浓度组对胃癌细胞株MGC803作用48h后,早期凋亡率分别为84.52％、55.58％、51.76％.(3)与阴性对照组相比,健脾消癥方各浓度组对胃癌细胞株MGC803作用48h后,可将细胞阻滞于G2-M期,健脾消癥方高浓度组在G0-G1期前出现较明显的亚二倍体峰.结论:(1)健脾消癥方可明显抑制胃癌细胞株MGC803的增殖作用.(2)健脾消癥方对人胃癌细胞株MGC803有明显的诱导凋亡及阻滞细胞周期在G2-M期的作用.
Abstract
Objective: To investigate the effect of Jianpi Xiaozheng formula on proliferation, apoptosis and cell cycle of gastric carcinoma cell line MGC803 in vitro. Methods: After gastric carcinoma cell line MGC803 was treated with Jianpi Xiaozheng Formula in different concentrations, cell proliferation was assessed using MTT assay. The apoptosis rate was examined by flow cytome-ter using Annexin V-FITC/PI double staining. Apoptosis and cell cycle were examined by flow cytometry ( FCM ). Results: Jianpi Xiaozheng Formula significantly inhibited the proliferation of MGC803 cells in a concentration-dependent manner. The apoptosis rate of high-concentration group, moderate-concentration group and low-concentration group in 24h were 49. 83% ,55. 29% ,16. 73% and in 48h were 84. 52% ,55. 58% ,51. 76% , respectively. As compared with the negative control group, when the cells were treated by high-concentration group,the percentage of the cells in G2-M phase increased in treatment group, which showed the cells were arrested at the G2-M phase of the cell cycle with the increased rate of cell apoptosis. Conclusion:Jianpi Xiaozheng Formula can inhabit the proliferation of MGC803 ,and can significantly induce apoptosis and arrest the cells at the G2-M phase of the cell cycle.
基金项目
江苏高校优势学科建设工程资助项目()
国家中医药管理局中医行业科研专项(201007007)
国家科技期刊平台
NSTL
维普
万方数据