Objective To establish a mouse model of non-alcoholic fatty liver disease related to psychological stress and to explore the therapeutic effect of Sini Powder(四逆散).Methods Forty male C57BL/6J mice were randomly divided into blank group(CON),high sucrose rich cholesterol diet group(CHR),compound model group(CS+CHR),paracetamol group(BLT)and Sini Powder group(SNS).Except CON group,all the other groups were given CHR diet,and CS+CHR group,BLT group and SNS group were randomly given two different kinds of stress every day for 60 days.BLT group and SNS group were given intragastric administration 30 days after the establishment of the model.The behavioral changes of mice were observed,the liver index,thymus index and fat index were calculated,the contents of total cholesterol(TC)and triglyceride(TG)in liver tissue were detected,the pathological changes of liver tissue were observed by oil red O and HE staining,and the contents of serum corticosterone(CORT),aldosterone(ALD),insulin(INS)and glucagon(GCG)were measured.Results Compared with those of the CON group,fat index and TC content of the CHR group increased,the upright times of the CS+CHR group decreased,fat index and the levels of TC,TG,CORT,ALD and GCG significantly increased.The upright times of the CS+CHR group decreased,the levels of TC,TG,ALD,INS and GCG significantly increased compared with those of the CHR group.Compared with those of the CS+CHR group,the upright times of the SNS Powder group increased,the contents of TG and ALD decreased significantly,and the fat index,the levels of TC content and ALD decreased significantly in the BLT group.Oil red O staining showed that a small number of lipid droplets could be seen in the CS+CHR group,and reduced in BLT group and SNS group,while HE paraffin staining showed that a small number of round vacuoles of different sizes and numbers were seen in the CHR group,and some round vacuoles were squeezed to one side of hepatocytes in the CS+CHR group,and a large number of lipid droplets were found in the CS+CHR group,and some liver nuclei were squeezed to one side of hepatocytes in BLT group and SNS group.Conclusion CUMS combined with CHR diet can better establish the model of psychological stress-related NAFLD mice.Sini Powder may improve the disease by reducing the content of serum aldosterone.
维普
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