Study on Activation of Wnt Signaling Pathway by Moxibustion and Intervention of Serum to Promote Endogenous Neural Stem Cell Proliferation and Differentiation in Rats with Spinal Cord Injury
Objective To explore the study of Lao moxibustion mediated Wnt signal pathway interfering with serum of rats with spinal cord injury to promote the proliferation and differentiation of endogenous neural stem cells.Methods Allen's method was used to prepare the rat model of spinal cord injury.They were divided into sham operation group,model group,Wnt inhibitor group,Lao moxibustion group,Lao moxibustion+Wnt inhibitor group.After 7 days of intervention,the serum was collected from the rats of each group.Three days after successful modeling,the spinal cord tissue of rats was isolated and cultured with endoge-nous neural stem cells.Serum intervention cells were used in each group,and the proliferation and differentiation status of cells in each group were observed 7 days later.Western Blot and qRT-PCR were used to detect the protein expressions and gene expres-sions of the markers of proliferation and differentiation of endogenous neural stem cells after the intervention of isolated serum in each group.Results Immunofluorescence staining showed that the proliferation and differentiation of endogenous neural stem cells into neurons were most significant in the Lao moxibustion group.The model group and Wnt inhibitor group showed the least prolif-eration and differentiation.The results of Western Blot and qRT-PCR showed that the Lao moxibustion group was the most signif-icant,while the difference between the Wnt inhibitor group and the model group was not significant.Conclusion Lao moxibustion activated Wnt signaling pathway in rats with spinal cord injury,promoted the proliferation and differentiation of endogenous neural stem cells into neurons and oligodendrocytes,inhibited the differentiation into astrocytes and made the repair and regeneration of nerve cells.
Wnt signal pathLao moxibustionserum of rats with spinal cord injuryendogenous neural stem cellsprolifera-tion and differentiation
万方数据
维普
