Xiao'ai Jiedu Prescription(消癌解毒方)Inhibits Growth of H22 Tumor-Bearing Mice and Regulates Bcl-2/CytC Signaling Pathway to Induce Apoptosis
Objective To investigate the effects of Xiao'ai Jiedu Prescription(消癌解毒方)on tumor growth and the expres-sions of key proteins in Bel-2/CytC signaling pathway in H22 tumor-bearing mice.Methods The H22 hepatocellular carcinoma transplantation tumor model was constructed.C57BL/6J mice were randomly divided into model group(normal saline by gavage),Xiao'ai Jiedu Prescription low-dose group(10 g·kg-1·d-1 gavage),Xiao'ai Jiedu Prescription medium-dose group(20 g·kg-1·d-1gavage),Xiao'ai Jiedu Prescription high-dose group(40 g·kg-1·d-1 gavage),cisplatin group(1 mg·kg-1·d-1,intraperitoneal injection)and blank group(normal saline by gavage),10 mice in each group.After 11 days of administration,the tumor weight was recorded and the tumor inhibition rate was calculated.Pathological changes were observed by Hematoxylin-eosin(HE)staining,the apoptosis rate was detected by TUNEL kit,and the expressions of JAK2 and STAT3 mRNA in tumor tissues were detected by Real-time PCR.The protein expressions of Bax,Bel-2,CytC,Caspase-3 and Cleaved Caspase-3 in tumor tissues were detected by Western blot.Results Xiao'ai Jiedu Prescription high,medium and low dose groups and cisplatin group could inhibit tumor growth in H22 tumor-bearing mice with the inhibitory rates of 59.9%,34.5%,21.5%and 67.1%,respectively.Compared with those in the model group,the pathological mitotic figures were reduced and tumor cells were sparsely arranged in the drug groups,showing a better inhibitory effect.The results of TUNEL showed that the tumor cells showed typical apoptotic signs after Xiao'ai Jiedu Prescription treatment,especially in the high-dose group.Rt-PCR showed that JAK2 and STAT3 mRNA expressions were down-regulated in each dose group of Xiao'ai Jiedu Prescription.Western blot as-say showed that Xiao'ai Jiedu Prescription could up-regulate the expressions of Bax,CytC,Cleaved Caspase-3 protein and down-regulate the expression of Bcl-2 protein in H22 tumor-bearing mice,and finally activate Caspase-3 protein to induce apoptosis of tumor cells.Conclusion Xiao'ai Jiedu Prescription can inhibit the growth of H22 tumor-bearing mice and induce the apoptosis of H22 cells,which may be related to the regulation of the expressions of key proteins in the Bcl-2/CytC signaling pathway,so as to play an anti-tumor role.