首页|响应面法优化"水城春"绿茶茶多酚的冲泡条件及其抗氧化活性

响应面法优化"水城春"绿茶茶多酚的冲泡条件及其抗氧化活性

The Optimization of Brewing Conditions and Antioxidant Activity of Polyphenols from Shuichengchun Green Tea Using Response Surface Method

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以"水城春"绿茶为原料,采用响应面法优化"水城春"绿茶茶多酚的冲泡条件,并测定其对DPPH·和ABTS+·的清除率,评价最佳冲泡条件下"水城春"绿茶茶多酚的抗氧化活性.结果表明:"水城春"绿茶茶多酚最佳冲泡条件为冲泡3次,100 mL水中茶添加量0.554 2 g、冲泡时间12 min、冲泡温度92℃;在此条件下,茶多酚溶出率为14.52%,与预测值14.60%接近,茶多酚提取率为73.33%.当"水城春"绿茶茶多酚浓度为16.18 μg/mL时,其对DPPH·和ABTS+·的清除率分别为90.23%、91.80%,清除能力均优于同浓度Vc溶液.由此可见,"水城春"绿茶具有较好的抗氧化活性.
To evaluate the antioxidant activity of tea polyphenols in Shuichengchun green tea under optimal brewing condi-tions.Using commercially available Shuichengchun green tea as raw material,the response surface methodology was used to opti-mize brewing conditions of polyphenols,and the scavenging rates of DPPH·and ABTS+·were measured.The results showed that the optimal brewing conditions for Shuichengchun green tea were 3 times,with a tea addition of 0.554 2 g in 100 mL of water,a brewing time of 12 minutes,and a brewing temperature of 92℃.Under the conditions,the dissolution rate of tea polyphenols was 14.52%,which was close to the predicted value of 14.60%.The extraction rate of tea polyphenols reached 73.33%.The scaveng-ing rate of DPPH·and ABTS+·are 90.23%and 91.80%when the concentration of polyphenols is 16.18 μg/mL.It can be inferred that the polyphenols in Shuichengchun green tea have better scavenging abilities for DPPH·and ABTS+·than Vc solutions of the same concentration.Therefore,Shuichengchun green tea has good antioxidant activity.

Shuichengchun green teatea polyphenolsbrewing conditionsantioxidant activity

李金辉、张泽敏、孙蓉、罗帆、潘宇荷、翁贵英

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六盘水师范学院 化学与材料工程学院,贵州 六盘水 553004

六盘水师范学院 生物科学与技术学院,贵州 六盘水 553004

"水城春"绿茶 茶多酚 冲泡条件 抗氧化活性

贵州省教育厅高校科技创新团队项目六盘水师范学院大学生科研训练计划项目六盘水师范学院科技创新团队项目

黔教技[2023]088号2022DK027LPSSYKJTD201904

2024

六盘水师范学院学报
六盘水师范学院

六盘水师范学院学报

影响因子:0.271
ISSN:1671-055X
年,卷(期):2024.36(3)
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