响应面法优化"水城春"绿茶茶多酚的冲泡条件及其抗氧化活性
The Optimization of Brewing Conditions and Antioxidant Activity of Polyphenols from Shuichengchun Green Tea Using Response Surface Method
李金辉 1张泽敏 1孙蓉 1罗帆 1潘宇荷 1翁贵英2
作者信息
- 1. 六盘水师范学院 化学与材料工程学院,贵州 六盘水 553004
- 2. 六盘水师范学院 生物科学与技术学院,贵州 六盘水 553004
- 折叠
摘要
以"水城春"绿茶为原料,采用响应面法优化"水城春"绿茶茶多酚的冲泡条件,并测定其对DPPH·和ABTS+·的清除率,评价最佳冲泡条件下"水城春"绿茶茶多酚的抗氧化活性.结果表明:"水城春"绿茶茶多酚最佳冲泡条件为冲泡3次,100 mL水中茶添加量0.554 2 g、冲泡时间12 min、冲泡温度92℃;在此条件下,茶多酚溶出率为14.52%,与预测值14.60%接近,茶多酚提取率为73.33%.当"水城春"绿茶茶多酚浓度为16.18 μg/mL时,其对DPPH·和ABTS+·的清除率分别为90.23%、91.80%,清除能力均优于同浓度Vc溶液.由此可见,"水城春"绿茶具有较好的抗氧化活性.
Abstract
To evaluate the antioxidant activity of tea polyphenols in Shuichengchun green tea under optimal brewing condi-tions.Using commercially available Shuichengchun green tea as raw material,the response surface methodology was used to opti-mize brewing conditions of polyphenols,and the scavenging rates of DPPH·and ABTS+·were measured.The results showed that the optimal brewing conditions for Shuichengchun green tea were 3 times,with a tea addition of 0.554 2 g in 100 mL of water,a brewing time of 12 minutes,and a brewing temperature of 92℃.Under the conditions,the dissolution rate of tea polyphenols was 14.52%,which was close to the predicted value of 14.60%.The extraction rate of tea polyphenols reached 73.33%.The scaveng-ing rate of DPPH·and ABTS+·are 90.23%and 91.80%when the concentration of polyphenols is 16.18 μg/mL.It can be inferred that the polyphenols in Shuichengchun green tea have better scavenging abilities for DPPH·and ABTS+·than Vc solutions of the same concentration.Therefore,Shuichengchun green tea has good antioxidant activity.
关键词
"水城春"绿茶/茶多酚/冲泡条件/抗氧化活性Key words
Shuichengchun green tea/tea polyphenols/brewing conditions/antioxidant activity引用本文复制引用
基金项目
贵州省教育厅高校科技创新团队项目(黔教技[2023]088号)
六盘水师范学院大学生科研训练计划项目(2022DK027)
六盘水师范学院科技创新团队项目(LPSSYKJTD201904)
出版年
2024
国家科技期刊平台
NSTL
万方数据