Genome Sequencing and Analysis of Alternaria tenuissima P1-2W Causing'Hongyang'Kiwifruit Soft Rot
To understand the pathogenicity mechanism of pathogens,we reported the genome sequence of Alternaria tenuissi-ma P1-2W as a causal agent of soft rot of'Hongyang'kiwifruit by a combination of Illumina NovaSeq and PacBio SMRT sequenc-ing methods in this study.The result indicated that the genome of P1-2W is approximately 33.87 Mb in size with an N50 value of 6.8 Mb and a GC content of 48.6%.The genome consists of 10 scaffolds,in which 5 ones exhibited the telomere repeat sequences(TTAGGG)at both ends,while the other 5 only at one end.The total of 11 726 genes were predicted,including 34 ncRNAs,118 tRNAs,25 rRNAs and 11 549 protein-coding genes(PCGs).The functional annotations of these PCGs based on COG,GO and KEGG suggested that"carbohydrate transport and metabolism"was very important.In addition,a total of 569 predicted carbohy-drate active enzymes(CAZymes)were annotated.Information obtained from this study enhances our knowledge about this patho-gen and will facilitate further research on its pathogenicity mechanism.
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