'红阳'猕猴桃软腐病病原菌细极链格孢菌的基因组组装与功能分析
Genome Sequencing and Analysis of Alternaria tenuissima P1-2W Causing'Hongyang'Kiwifruit Soft Rot
陈玲玲 1张书东 2邰思涵 2罗兰英 2凌立贞2
作者信息
- 1. 大连大学生命健康学院,辽宁 大连 116622;六盘水师范学院贵州西部民族药用植物资源研究与利用重点实验室,贵州 六盘水 553004
- 2. 六盘水师范学院贵州西部民族药用植物资源研究与利用重点实验室,贵州 六盘水 553004
- 折叠
摘要
以'红阳'猕猴桃软腐病病原菌细极链格孢菌为材料,利用三代结合二代测序技术对其进行基因组测序.结果表明,组装的细极链格孢菌基因组大小为33.87 Mb,N50长度为6.8 Mb,GC含量为50.87%.基因组的组装共产生10条scaffolds,其中5条scaffolds两端包含端粒重复序列(TTAGGG),而另外5条仅一端有端粒重复序列.预测的11 726个基因包括34个ncRNAs、118个tRNAs、25个rRNAs和11 549个编码基因.对编码基因进行COG、GO和KEGG功能注释,表明碳水化合物的转运和代谢相关基因发挥重要功能.此外,发现有569个碳水化合物活性酶(CAZymes)基因.这些结果可为解析细极链格孢菌的致病机制提供基础数据.
Abstract
To understand the pathogenicity mechanism of pathogens,we reported the genome sequence of Alternaria tenuissi-ma P1-2W as a causal agent of soft rot of'Hongyang'kiwifruit by a combination of Illumina NovaSeq and PacBio SMRT sequenc-ing methods in this study.The result indicated that the genome of P1-2W is approximately 33.87 Mb in size with an N50 value of 6.8 Mb and a GC content of 48.6%.The genome consists of 10 scaffolds,in which 5 ones exhibited the telomere repeat sequences(TTAGGG)at both ends,while the other 5 only at one end.The total of 11 726 genes were predicted,including 34 ncRNAs,118 tRNAs,25 rRNAs and 11 549 protein-coding genes(PCGs).The functional annotations of these PCGs based on COG,GO and KEGG suggested that"carbohydrate transport and metabolism"was very important.In addition,a total of 569 predicted carbohy-drate active enzymes(CAZymes)were annotated.Information obtained from this study enhances our knowledge about this patho-gen and will facilitate further research on its pathogenicity mechanism.
关键词
'红阳'猕猴桃/软腐病/细极链格孢菌/基因组/碳水化合物活性酶Key words
Alternaria tenuissima/genome/'Hongyang'kiwifruit/post-harvested rot disease/CAZymes引用本文复制引用
基金项目
六盘水市科技发展项目(52020-2023-02-18)
六盘水师范学院大学生创新训练项目(S202310977123)
&&(S202310977124)
出版年
2024
国家科技期刊平台
NSTL
万方数据