Application of 454 high-throughput Sequencing Method in Soil Microorganism
Soil microorganisms are the important components of soil ecosystem and play an important role in biogeochemical cycling such as organic matter decomposition ,mineral nutrient release and energy transformation . There are many types of soil microorganisms which have been in the study .From the original plate culture method to today's high -throughput sequencing method ,it has great changes in the study of soil microbial technology .The result is getting more and more accurate as the technology has been improved bit by bit .The high - throughput sequencing method can test the sequence of tens of thousands of strands of DNA ,which can greatly reduce the time and cost of technology . This article briefly analyzes high - throughput sequencing method , and focuses on the application of the new generation of sequencing technology in the soil microorganisms .