GC-MS Analysis of Rhizospiere—Soil Extract of Pinus massoniana Seedings
In order to provide scientific basis and theoretical guidance for natural regeneration and soil deg-radation,the two years of Pinus massoniana seedings were used as the test plant to analyze the variety and content of the extracts from rhizospheric soil of Pinus massoniana seedlings.Ethanol and methanol extracts were used to extract and inoculate the rhizosphere soils of the seedlings.Gas chromatography-mass spectrometry techniques was used in material identification.The results showed that thirty—seven compounds were identified from methanol extracts in the rhizosphere soil of Pinus massoniana;forty—six compounds were identified by ethanol extracts.The rhizospheric soil extracts(extracted with methanol)of Pinus massoniana seedlings had 9 more components than those of extracted with ethanol.The relative contents of chemical constitute was less 14.16%.Under the treatment of two extraction methods,the relative contents of chemical constitute were both esters,alcohols and acids,but the proportion of various compounds are different form each other.The palmitic acid,carboxylic acid and oleic acid were the acids which detected under the treatment of methanol and ethanol.In terms of the composition of extracts,the rhizosphere soil extracts of Pinus massoniana contain a variety of organic compounds,mainly acids,es-ters,alcohols,hydrocarbons,aldehydes,phenols and other substances.The relative content of each compound is different,and the types and relative content of different compounds have important ecologi-cal significance for the growth of Pinus massoniana.However,the interaction mechanism between the material types for the growth of Pinus mnassoniana and natural regeneration remains to be further in—depth study.