Eucalyptus cinerea is an important leaf cutting material in the market of fresh cut flowers at home and abroad.Screening the best medium for the growth and rooting of its stem tips and establishing its tissue culture and rapid propagation system can provide a reference for the factory production of its tissue culture seedlings.U-sing the aseptic stem tip of E.cinerea as experimental material,the growth and rooting under different basic medi-um,6-BA,NAA combination and its concentration were analyzed,and the tissue culture seedlings were trans-planted.The results showed that basic medium was the main factor affecting the browning and proliferation of E.cinerea tissue culture seedlings,and NAA was the main factor affecting the rooting.Under the combined treat-ment of 1/4WPM+6-BA 1.3 mg/L+NAA 0.1 mg/L,the browning rate(2.63％)of the stem tips was lower;the proliferation rate(115.79％)and rooting rate(97.37％)were the highest,and the seedlings were robust and the leaf color was dark green,and the survival rate of transplanting was 73.71％.It can be seen that 1/4WPM+6-BA 1.3 mg/L+NAA 0.1 mg/L+Sucrose 15 g/L+Agar 6.0 g/L as the best medium can establish an efficient tissue cul-ture and rapid propagation system of stem tip of Eucalyptus cinerea.
关键词
尤加利/茎尖/组织培养/快繁体系
Key words
Eucalyptus cinerea/stem tips/tissue culture/rapid propagation system
维普
万方数据