Establishment of Tissue Culture and Rapid Propagation System of Eucalyptus cinerea
Eucalyptus cinerea is an important leaf cutting material in the market of fresh cut flowers at home and abroad.Screening the best medium for the growth and rooting of its stem tips and establishing its tissue culture and rapid propagation system can provide a reference for the factory production of its tissue culture seedlings.U-sing the aseptic stem tip of E.cinerea as experimental material,the growth and rooting under different basic medi-um,6-BA,NAA combination and its concentration were analyzed,and the tissue culture seedlings were trans-planted.The results showed that basic medium was the main factor affecting the browning and proliferation of E.cinerea tissue culture seedlings,and NAA was the main factor affecting the rooting.Under the combined treat-ment of 1/4WPM+6-BA 1.3 mg/L+NAA 0.1 mg/L,the browning rate(2.63％)of the stem tips was lower;the proliferation rate(115.79％)and rooting rate(97.37％)were the highest,and the seedlings were robust and the leaf color was dark green,and the survival rate of transplanting was 73.71％.It can be seen that 1/4WPM+6-BA 1.3 mg/L+NAA 0.1 mg/L+Sucrose 15 g/L+Agar 6.0 g/L as the best medium can establish an efficient tissue cul-ture and rapid propagation system of stem tip of Eucalyptus cinerea.
Eucalyptus cinereastem tipstissue culturerapid propagation system
万方数据
维普
