摘要
为探究Cd胁迫下三色堇生长、光合特性及其生理响应的变化,采用盆栽实验,测定生长在不同浓度(0、5、10、20、50、100、200 mg/kg)Cd的栽培基质中三色堇的生长、光合及抗逆生理指标的变化,以期为三色堇的Cd耐受性、在Cd污染土壤的应用及潜在的Cd污染修复植物筛选提供理论依据.结果表明:低浓度(5～10 mg/kg)下,三色堇的生长指标、净光合速率、蒸腾速率略有增加,并可见可溶性蛋白、可溶性糖和游离脯氨酸含量增加以及SOD、POD、CAT的激活,表现出一定的Cd耐受能力;当Cd浓度较高时(≥100 mg/kg),三色堇表现出较明显的毒害表现,包括生长与光合抑制、SPAD值下降、MDA含量显著增加、酶促保护系统活性和渗透调节物质含量下降.胁迫时间的延长总体起着加深胁迫的作用.
Abstract
In order to explore the changes in the growth,photosynthetic characteristics and physiological responses of Viola tricolor L.under Cd stress,this study used a pot experiment to measure the changes in growth,photosyn-thesis and stress-resistance physiological indicators of Viola tricolor L.grown in different Cd concentrations(0,5,10,20,50,100 and 200 mg/kg),which expected to provide a theoretical basis for the Cd tolerance determination of Viola tricolor L.,its application in Cd-contaminated soil and the screening of potential Cd-contaminated remedia-tion plants.The results showed that at low concentrations(5～10 mg/kg),the growth indicators,net photosynthetic rate,and transpiration rate of Viola tricolor L.increased slightly,and the content of soluble protein,soluble sugar,and free proline increased as well as SOD,POD,and CAT activation,showing a certain degree of Cd tolerance;when the Cd concentration is high(≥100 mg/kg),Viola tricolor L.shows obvious toxic effects.Growth and pho-tosynthesis inhibited;SPAD value decreased;MDA content increased significantly;enzymatic protection system activity and osmotic adjustment substance content decreased.The prolongation of the stress time generally plays the role of deepening the stress.
NETL
NSTL
维普
万方数据