Isolation,Identification and Degradation Characteristics of High Efficiency Diesel Degrading Bacteria
Two highly efficient petroleum-degrading strains,CY-1 and CY-2,were isolated and screened from soil contaminated with diesel fuel.Following morphological observation,physiological and biochemical experiments,as well as 16S rRNA gene sequence analysis,CY-1 was identified as Pseudomonas plecoglossicida while CY-2 was identified as Gordonia terrae.The optimal conditions for the degradation of diesel oil by these two high-effi-ciency bacteria strains and their metabolism of straight-chain alkanes were investigated.Experimental results dem-onstrated that at a culture temperature of 30 ℃,pH of 7.0,diesel oil concentration of 500 mg/L,inoculation a-mount of 8％,and NaCl concentration of 0.5％,the degradation rate achieved by CY-1 reached 65.51％after a seven-day culture period with an average degradation rate for C15-C27 alkanes reaching 64.30％.Similarly,when cultured at a temperature of 30℃ with an initial pH value of 7.0,a diesel oil concentration of 3000 mg/L,an inoc-ulation amount of 2％,and a NaCl concentration also at 0.5％,the diesel oil degradation rate exhibited by strain CY-2 reached up to approximately 64.87％after seven days with an average degradation rate for alkanes ranging from C14 to C27 reaching approximately 87.73％.Both strains have a certain application prospect in diesel pollu-tion control,and the diesel oil degradation performance of CY-2 strain was better than that of CY-1 strain.
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