Research on the Reuse of Huangshui Resources Based on Small Molecule Chemical Composition
This paper studied the small molecule chemical composition of Huangshui and explored the potential u-ses of Huangshui resources for reuse based on this.The content of amino acids in Huangshui are determined by u-sing ultra-high performance liquid chromatography coupled with mass spectrometry;The Huangshui was purified by molecular sieve,silica gel,OSD column chromatography and semi-preparative high performance liquid chro-matography,and the structure of the separated compounds was identified by modern spectrum technology.The effect of Huangshui extract on migration ability of L-929 cells was investigated by cell scratch test.The targeted determination results showed that Huangshui contains 19 types of amino acids,each of which is rich in content.Among them,serine,alanine,tyrosine,valine,and leucine have the highest content,reaching 3007.27,4883.83,3017.61,3362.24,5812.48,respectively μg/g;Two compounds were isolated and identified as 11-methyl-9-hydroxyl-8,9,10,11-tetrahydro,respectively-β-Carboline(1),1,2,3,4-tetrahydro-l-metal-β-Carboline-3carboxylic acid(2),both of which are amino acid derivatives;The cell scratch results showed that after 24 and 36 hours of treatment with Huangshui extract on L-929 cells,the scratch size significantly decreased and the cell migration a-bility significantly increased.It points out that Huangshui contains abundant amino acid components,and com-pounds 1 and 2 are the first amino acid derivative components isolated from Huangshui.Huangshui extract has a significant proliferative effect on L-929 cells,indicating that Huangshui extract has potential as a nutrient and provides new directions for resource utilization of Huangshui.
NETL
NSTL
万方数据
维普
