Due to its special biological characteristics,it is difficult to reproduce and the cost is high,which re-stricts the development of bamboo industry and makes it difficult to meet the market demand.The most effective solution at present is to obtain bamboo plant regeneration through tissue culture to achieve rapid propagation and cost reduction,improve breeding quality and obtain superior plants with hybrid advantage,as well as superior va-riety selection and mutant screening.The current research on bamboo histoculture shows that the most effective methods are through three ways:embryo culture,shoot multiplication by shoot,and healing tissue culture.Despite the successful construction of some bamboo species of histoponic fast propagation and efficient regeneration tech-nology system,there are still many problems to be solved.The tissue culture of loose bamboo has made no break-through in the establishment of in vitro rapid propagation and plant regeneration system,and it is difficult to es-tablish an efficient and stable rapid propagation and regeneration system.The suspension culture and protoplast culture system need to be further improved.The instability of bamboo flowering leads to the limited seed embryo material,which greatly limits the research of bamboo tissue culture.There is material browning,vitrification and pollution.Tissue culture of loose bamboo has not made a breakthrough in the establishment of isolated fast propa-gation and vegetative regeneration system.It is difficult to establish an efficient and stable fast propagation and regeneration system.Suspension culture and protoplasm culture system needs to be further improved.The instabil-ity of bamboo flowering has led to the limited availability of seed embryo material,which greatly limits the re-search on bamboo plant tissue culture.In this regard,the establishment of a tissue culture system corresponding to different bamboo species is an urgent problem that can only be effectively protected and exploited.With further research on flowering induction of bamboo seedlings in vitro and tissue culture technology,there will be break-throughs and substantial progress in the problems faced by bamboo biotechnology breeding,and the development of bamboo industry and ecological,economic and social benefits will be further improved.
bambootissue culturerapid propagationregeneration system
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