Research on Tissue Culture and Regeneration Technology of Dendropanax Dentiger
Dendropanax dentiger has a good development prospect in the fields of medicine,food and landscaping.Conventional seedling raising method has been unable to meet the market demand of Dendropanax dentiger seed-lings.In this paper,the tissue culture technology of Dendropanax dentiger was studied in order to provide a theo-retical basis for the factory seedling cultivation in the future.Healthy annual semi-lignified branches free of pests and diseases were selected as the explants.The effects of different medium types and hormone concentrations on the regeneration process of Dendropanax dentiger tissue culture were studied by means of single factor and or-thogonal design.The test results show that:MS medium was more suitable for the induction of adventitious buds than WPM medium.The best medium is MS+ZT1.5mg/L+6-BA1.5mg/L+NAA0.4mg/L;The multiplication rate and multiplication coefficient of improve MS+6-BA1.0mg/L+KT0.6mg/L+NAA0.5mg/L were the highest.The basic medium for rooting culture was 1/2MS.The best medium is 1/2MS+IBA1.0mg/L+ABT10.6mg/L+NAA 0.1 mg/L;Peat soil∶yellow soil∶perlite(2∶1∶1)was suitable as the transplanting medium,and the survival rate of transplanting was 89.8％.The following conclusions can be drawn:High concentration of inorganic salt i-ons can promote the germination of adventitious buds,but also increase the possibility of glass transition;cytokin-ins ZT and 6-BA play important roles in the induction and proliferation of adventitious buds;high concentration of 6-BA can inhibit the proliferation of adventitious buds;auxin NAA had little effect on adventitious root forma-tion.
NETL
NSTL
万方数据
