Identification of CsTRPSGene in Camellia sinensis and Analysis of Its Expression Under Pea-Camellia sinensis Intercropping
TRPs(tryptophan synthase beta chain 1)gene,as a precursor to the synthesis of indole acetic acid,regu-lated the synthesis of tryptophan,which is widely existed in various plants,and affected the growth of plants by regulating the synthesis of auxin.However,studies on TRPs genes in tea plants have not been reported.Therefore,this study took tea tree as experimental material,cloned CsTRPS gene of Camellia sinensis,and analyzed CsTRPS gene bioinformatically.The transcriptome data were used to investigate the gene expression in the pea-Camellia sinensis intercropping.The results showed that the total length of CsTRPS gene was 1425 bp,encoding 474 amino acids.The molecular weight of CsTRPS protein was 51.552865 KDa;the theoretical isoelectric point(PI)was 6.52;the content of glycine(Gly)residue was the highest,accounting for 10.1％,and it was a hydrophilic protein with-out signal peptide and transmembrane structure.In the secondary structure of CsTRPS protein,a-helix accounted for 38.19％;β-angle accounted for 7.59％;extended chain accounted for 17.09％,and irregular curling accounted for 37.13％.Subcellular localization prediction showed that CsTRPS protein was mainly expressed in mitochon-dria.Phylogenetic tree showed that CsTRPS protein of Camellia sinensis had the closest evolutionary relationship with RvTRPS protein of Rhododendron vialii Delavay et Franch.,AcTRPS protein of kiwifruit,and D1TRPS pro-tein of Diospyros lotus L..Transcriptome data showed that the expression of CsTRPS gene in Camellia sinensis plant was down-regulated under pea-Camellia sinensis intercropping conditions.
Camellia sinensisCsTRPS genebioinformatics analysispea and Camellia sinensis intercropping
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NSTL
万方数据
