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云南蓝莓根腐病病原菌鉴定初报

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为明确引起云南蓝莓根腐病的病原菌种类,利用常规组织分离纯化方法对患病的蓝莓植株根部进行病原菌分离和纯化,利用柯赫氏法则验证疑似病原菌菌株的致病性,然后结合菌株的形态学特征和ITS序列系统发育分析进行了种类鉴定.结果表明:共享镰刀菌(Fusarium commune)可引起蓝莓根腐病的发生,这是该病在云南省的首次报道.该病原菌菌丝生长最适温度为25~30 ℃,最适碳源为淀粉和乳糖,最适氮源为牛肉膏、硝酸钾和蛋白胨,最适生长pH值为6~9,具有极强的生存能力.研究结果为云南蓝莓根腐病的综合防治提供了理论依据.
Preliminary Report on the Identification of the Pathogen Causing Root Rot on Blueberry in Yunnan
In order to clarify the species of pathogenic bacteria causing blueberry root rot in Yunnan,this study used conventional tissue separation and purification methods to isolate and purify the pathogenic bacteria from the diseased blueberry roots,and used Koch's rule to verify the pathogenicity of the suspected pathogenic bacterial strains.The species are identified by combining the morphological characteristics of the strains and ITS sequence phylogenetic analysis.The results showed that Fusarium commune could cause blueberry root rot,which was first reported in Yunnan Province.The optimal temperature for mycelia growth of the pathogen was 25~30 ℃.The optimal carbon source was starch and lactose.The optimal nitrogen source was beef paste,potassium nitrate and peptone.The optimal pH was 6~9,and it had a strong survival ability.The results provided a theoretical basis for the comprehensive control of Yunnan blueberry root rot.

blueberry root rotpathogen identificationFusarium commune

沈艳、祝友朋

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大方县农业农村局,贵州 大方 551700

毕节市林业局,贵州 毕节 551700

蓝莓根腐病 病原菌鉴定 共享镰刀菌

2024

绿色科技
花木盆景杂志社

绿色科技

影响因子:0.365
ISSN:1674-9944
年,卷(期):2024.26(7)
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