植物提取物0.3％SN-016水剂防治根结线虫生物活性测定
Bioactivity Assays of Plant Extracts 0.3％SN-016 AS on Controlling Root-knot Nematodes
王炳太 1王伟 2孙砚芬 3郭建慧 4曹秀花 5孟凤新 6李德福 7窦传峰2
作者信息
- 1. 潍坊职业学院,山东 潍坊 262737;潍坊市农业科学院,山东 潍坊 261071;潍坊市根结线虫防控工程技术研究中心,山东 潍坊 262737
- 2. 高密市农业技术推广中心,山东高密 261500
- 3. 潍坊市根结线虫防控工程技术研究中心,山东 潍坊 262737
- 4. 潍坊职业学院,山东 潍坊 262737;潍坊市根结线虫防控工程技术研究中心,山东 潍坊 262737
- 5. 安丘市大汶河旅游发展中心,山东安丘 262100
- 6. 潍坊市昌乐县行政审批服务局,山东 昌乐 262400
- 7. 潍坊市农业农村局,山东 潍坊 261061
- 折叠
摘要
为了筛选高效、低毒、安全、无残留、环境友好型的新型植物源杀线虫剂,潍坊植物病原线虫联合研究团队项目组进行了植物提取物0.3％SN-016水剂(AS)防治根结线虫生物活性测定.项目组采用"Z"字形五点采样法,在试验地采集黄瓜根结线虫、番茄根结线虫、西瓜根结线虫土样.在实验室,项目组采用离心悬浮法,结合卡勃(Cobb)过筛分离法,分离出南方根结线虫(Meloidogyne incognita Chitwood)侵染性二龄幼虫.测定结果表明:在处理1(浓度8 g/L))、处理2(浓度4 g/L)、处理3(浓度2 g/L)、处理4(浓度1 g/L)、处理5(浓度0.5 g/L)中,侵染性二龄幼虫死亡率平准值分别为97.18％、81.74％、44.39％、14.62％、8.81％.指出了在处理1(浓度8 g/L))和处理2(浓度4 g/L)中,侵染性二龄幼虫的死亡率平准值分别为97.18％、81.74％,这表明植物提取0.3％SN-016水剂(AS)对黄瓜根结线虫、番茄根结线虫、西瓜根结线虫侵染性二龄幼虫有良好的生物活性.
Abstract
In order to screen efficient,low toxicity,safe,residue-free and environmentally friendly new plant ori-gin nematicides,the project team of the Weifang Plant Pathogenic Nematode Joint Research Team conducted bio-activity assays of the plant extracts 0.3％SN-016 AS on controlling root-knot nematodes.The project team used the"Z"shaped five-point sampling method to collect soil samples of Cucumber Root-knot Nematodes,Tomato Root-knot Nematodes and Watermelon Root-knot Nematodes at the experimental sites.In the laboratory,the pro-ject team used the centrifugal suspension method,combined with Cobb sieving separation method,to separate the infecting second-instarred larvae of Meloidogyne incognita Chitwood.The results show that the average mortality rates of infecting second-instarred larvae were respectively 97.18％,81.74％,44.39％,14.62％and 8.81％in the treatment 1(concentration 8g/L),treatment2(concentration o4g/L),treatment 3(concentration 2g/L),treatment 4(concentration 1g/L)and treatment 5(concentration 0.5g/L).The conclusion is that the average mortality rates of infecting second-instarred larvae were respectively 97.18％and 81.74％in treatment 1(concentration 8g/L)and treatment 2(concentration 4g/L).This indicates that the plant extracts 0.3％SN-016 AS has a good bioactivity a-gainst infecting second-instarred larvae of Cucumber Root-knot Nematodes,Tomato Root-knot Nematodes and Watermelon Root-knot Nematodes.
关键词
植物提取物0.3％SN-016水剂/防治根结线虫/生物活性测定Key words
plant Extracts 0.3％ SN-016 AS/controlling root-knot Nematodes/bioactivity assays引用本文复制引用
出版年
2024
NETL
NSTL
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