Optimization of Reducing Sugar Parameters for Starch Extraction From α-amylase Hydrolyzed Potato
This study was optimized by using a single factor experiment method and a response surface method to optimize the amount of amzyla amount,pH,temperature and time of the amyloid hydrolysis potato.The optimum combination conditions were obtained by the response surface method of pH 6.0,and the enzyme was added 37 mg(0.37%).The enzymatic hydrolysis time was 5.1 h,and the enzymatic solution was 66 ° C.In this process,the degree of hydrolysis of potato is maximized.The extraction rate of reducing sugar was 5.104%and the error be-tween the extraction rate of reducing sugar and the predicted value of 5.20%is less than 2%.The results of enzy-molysis kinetics test showed that the change of enzymolysis rate was consistent with the characteristics of Mi e-quation under different substrate concentrations.The Km and Vmax were calculated by the method of double recip-rocal drawing which were 99.13 g/L and 2.92 g/(L·h)separately.The Michaelis Equation is V=2.92[S]/(99.13+[S])and R2=0.99752.The equation was very significant which could be used to simulate the enzymol-ysis kinetic process of α-amylase.The research could provide a reference for resource-based potato waste.
α-amylaseresponse surface methodenzymatic hydrolysis kineticextraction of reducing sugarMi-chael's equation
万方数据
维普
