Three chemical constituents in Rubus sachalinensis are simultaneously determined by using quantitative analysis of multi-components by single-marker(QAMS).An high performance liquid chromatography(HPLC)method was used.The determination was performed on SB-Aq-C18(250 mm× 4.6 mm,5 μm)with acetonitrile-0.1％phosphoric acid(17∶83,V/V)as the mobile phase.The column temperature,detection wavelength,flow rate and injection volume were 30 ℃,230 nm,1.0 mL/min,10 μL,respectively.With caffeic acid as the internal stand-ard,the relative correction factors of ellagic acid and hyperoside were calculated,and then the content determina-tion were carried out.The standard curves of caffeic acid,ellagic acid and hyperoside all showed good linearity within 0.18～1.85 μg(r=0.9997),0.062～0.62 μg(r=0.9998),0.03～0.30 μg(r=0.9993)respectively.The aver-age sample recovery range was 97.66％～98.23％.RSD range was 1.77％～2.25％(n=9)with the RSD＜3.0％.The established QAMS method can be used for simultaneously determining the contents of 3 components in Tibet-an medicine Rubus sachalinensis,and the method is simple,effective and accurate.
关键词
一测多评法/悬钩木/咖啡酸/鞣花酸/金丝桃苷/含量测定
Key words
quantitative analysis of multi-component by single marker/Rubus sachalinensis Lévl./caffeic acid/ellagic acid/hyperoside/content determination
维普
万方数据