首页|新立河水域撅嘴鲢与鲫鱼肠道微生物群落结构特征分析

新立河水域撅嘴鲢与鲫鱼肠道微生物群落结构特征分析

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利用高通量测序技术,分析了位于滨州市新立河水域中的撅嘴鲢与鲫鱼肠道菌群结构特征,及其与生活环境中微生物群落之间的关系.研究发现3种样品共鉴定出215个共有OTUs,撅嘴鲢、鲫鱼和水体样中特有的OTUs数目分别为843、1120和1168.撅嘴鲢和鲫鱼样本肠道菌群多样性和结构与周围水体存在显著差异,水体中的细菌丰富度和均匀度更高,细菌类型也更稳定.在门水平上,撅嘴鲢以变形菌门、厚壁菌门和梭杆菌门为优势菌门,鲫鱼以变形菌门、厚壁菌门和蓝菌门占优势,水体样本则以变形菌门、放线菌门和拟杆菌门为主;在目水平上,撅嘴鲢优势菌目为气单胞菌目、肠杆菌目和梭杆菌目,鲫鱼优势菌目为弧菌目、根瘤菌目和气单胞菌目,而水体中放线菌目、黄杆菌目和红杆菌目则为优势菌目.研究结果可为深入了解2种鱼类的肠道微生物群落结构组成,及其与外部环境之间的关系奠定了基础.
Analysis on the Structures of Intestinal Microbial Community of Chanodichthys dabryi and Carassius auratus in Xinli River
In this experiment,high-throughput sequencing technique was used to analyze the characteristics of in-testinal microbial community structure of pouting silver carp(Chanodichthys dabryi)and crucian carp(Carassius carassius)and their relationship with surrounding environment microbial community in Xinli River,Binzhou City,Shandong Province.It was found that a total of 215 common OTUs were identified in the three samples,and the unique OTUs numbers in pouting silver carp,crucian carp and water samples were 843,1120 and 1168 respectively.The diversity and structure of bacterial community in the intestines of pouting silver carp and crucian carp were significantly different from those in the surrounding water.The richness and evenness of bacteria in the water were higher,and the types of bacteria were more stable.At the gate level,the dominant phyla of silver carp were Proteus,Clostridium and Clostridium.Carassius auratus were dominant,and the water samples were mainly Pro-teus,actinomycetes and Bacteroides.At the eye level,the dominant bacteria of silver carp are Aeromonas,Enter-obacteriaceae and Clostridium.Carassius auratus are Vibrio,Rhizobium and Aeromonas,while actinomycetes,Fla-vobacterium and Rhodococcus are dominant bacteria in water.The results lay a foundation for an in-depth under-standing of the intestinal microbial community structure of the two species of fish and their relationship with the external environment.

fishXinli River16SrRNAgut microorganism

崔庆华、李建霞、梁照东、张兴安、张海峰、张一博

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滨州市环境保护科学技术研究所,山东滨州 256600

沾化区生态环境监控中心,山东 沾化 256800

滨州市生态环境服务中心,山东 滨州 256600

鱼类 新立河 16SrRNA 肠道微生物

2024

绿色科技
花木盆景杂志社

绿色科技

影响因子:0.365
ISSN:1674-9944
年,卷(期):2024.26(22)