On August 6,2021,a wild fungus was collected from the trunk of a willow tree in Liupanshan National Forest Park,Jingyuan County,Guyuan City,Ningxia Hui Autonomous Region.The morphological characteristics of the fruiting body of the fungus were observed.Its ITS sequence was amplified and sequenced.A phylogenetic tree was constructed.Referring to the appearance of Fomes fungarius recorded in relevant books,the wild fungus was identified as Basidiomycetes,Polyporales,Polyporaceae,Fomes,and Fomes fomentarius L ex.Fr;fermentation broth and mycelium of Fomes fomentarius is prepared,and antioxidant activity is determined.The mycelium and fermentation broth of Fomes fomentarius L ex.Fr showed certain differences in their ability to resist superoxide free radical oxidation and hydroxyl free radical oxidation,and their clearance rates were in the order of fermenta-tion broth>mycelium;The scavenging effect on DHHP free radicals and Fe3+reducing power are both higher in mycelium than in fermentation broth,with significant differences in the scavenging effect on DHHP free radicals.Overall,the antioxidant activity of mycelium is higher than that of fermentation broth.
关键词
木蹄层孔菌/菌丝体/发酵液/抗氧化活性
Key words
Fomes fomentarius L ex.Fr/mycelium/fermentation broth/antioxidant activity