首页|Enzymes immobilized in wood-derived cellulose scaffold for constructing a novel modular bioreactor

Enzymes immobilized in wood-derived cellulose scaffold for constructing a novel modular bioreactor

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Modular bioreactors can provide a flexible platform for constructing complex multi-step pathways,which may be a solution for maximizing reactions and overcoming the complexity of multi-enzyme systems.Here,we selected wood-derived cellulose scaffold as a support for enzyme immobilization and constructed the modular bioreactor.Cellulose scaffold was prepared after removing lignin from wood,followed by citric acid functionalization and the addition of glutaraldehyde finally allowed the cross-linking of enzymes.Three enzymes,horseradish peroxidase(HRP),glucose oxidase(GOD),and catalase(CAT),were separately immobilized,resulting in the immobilized enzyme amount to over 40 mg/g.The introduction of carboxyl groups from citric acid facilitated the rapid enzyme adsorption on the support surface and immobilized enzymes possess～65％expressed activity.Modular bio-reactors were constructed by using the immobilized enzymes.With the immobilized HRP module,reactor showed desired catalytic performance with the phenol degradation rate of＞90％.Also,a pH regulation can occur in the bioreactors for preserving enzyme activities and neutralizing acid products.In the GOD/CAT modular bioreactor,the cascade reaction with adjusting pH values can achieve a 95％yield of sodium gluconate and exhibit a favorable reusability of 5 operation cycles.

WoodImmobilized enzymeCitric acidModular bioreactorCascade reaction

Zhihong Zhang、Mengchen Jin、Guiru Chen、Jiandu Lei、Luying Wang、Jun Ge

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Beijing Key Laboratory of Lignocellulosic Chemistry,Beijing Forestry University,Beijing,100083,China

MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy,Beijing Forestry University,Beijing 100083,China

Key Lab for Industrial Biocatalysis,Ministry of Education,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China

Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen,518055,China

Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen,518107,China

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National Key Research and Development Program of ChinaBeijing Natural Science FoundationNational Natural Science Foundation of China

2021YFC2102804220203421978024

2023

绿色化学工程(英文)
中国科学院过程工程研究所

绿色化学工程(英文)

CSCD
ISSN：
年,卷(期)：2023.4(1)
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