首页|Engineering Escherichia coli for high-yield production of ectoine

Engineering Escherichia coli for high-yield production of ectoine

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Ectoine is a natural macromolecule protector and synthesized by some extremophiles.It provides protections against radiation-mediated oxidative damages and is widely used as a bioactive ingredient in pharmaceutics and cosmetics.To meet its growing commercial demands,we engineered Escherichia coli strains for the high-yield production of ectoine.The ectABC gene cluster from the native ectoine producer Halomonas elongata was intro-duced into different Escherichia coli(E.Coil)strains via plasmids and 0.8 g L-1 of ectoine was produced in flask cultures by engineered E.coli BL21(DE3).Subsequently,we designed the ribosome-binding sites of the gene cluster to fine-tune the expressions of genes ectA,ectB,and ectC,which increased the ectoine yield to 1.6 g L-1.After further combinatorial overexpression of Corynebacterium glutamicum aspartate kinase mutant(G1A,C932T)and the H.elongate aspartate-semialdehyde dehydrogenase to increase the supply of the precursor,the titer of ectoine reached to 5.5 g L1 in flask cultures.Finally,the engineered strain produced 60.7 g L1 ectoine in fed-batch cultures with a conversion rate of 0.25 g/g glucose.

EctoineEscherichia coliMetabolic engineeringRBS design

Daoan Wang、Jiamin Chen、Yang Wang、Guocheng Du、Zhen Kang

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The Science Center for Future Foods,Jiangnan University,Wuxi,214122,China

The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,214122,China

The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi,214122,China

National Key Research and Development Program of ChinaJiangsu Province Natural Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of ChinaKey Technologies R&D Program of Jiangsu ProvinceNational Firstclass Discipline Program of Light Industry Technology and Engineering

2021YFC2100800BK2020002532000058BE2019630LITE2018-16

2023

10.1016/j.gce.2021.09.002

绿色化学工程(英文)
中国科学院过程工程研究所

绿色化学工程(英文)

CSCD
ISSN：
年,卷(期)：2023.4(2)
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