首页|Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production

Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production

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Cadaverine is the key monomer for the synthesis of nylon 5X.Efficient and alkaline stable lysine decarboxylases are highly desirable for cadaverine production as the reaction pH increasing from 6.3 to 8.5.However,the most studied lysine decarboxylase CadA(E.coli)lost almost all activity at pH 8.0,which is the foremost challenge for the industrial-cadaverine production.In this study,we first found that the Na+-microenvironment significantly improved the alkaline stability of the disulfide engineered lysine decarboxylase △LdcEt3(P233C/L628C)(half-life 362 h),compared to the conventional buffer(half-life 0.66 h)at pH 8.0.Meanwhile,the whole-cell conversion efficiency of the industrial-grade L-lysine with △LdcEt3 could reach up to 99％in 2 h in the fermenter.Experi-mental investigation and molecular dynamics confirmed that Na+-microenvironment could improve active-aggregation state and affect secondary structure of △LdcEt3.Therefore,Na+-microenvironment stabilizes △LdcEt3 providing a great potential industrial application for high-level cadaverine production.

Na+-microenvironmentLysine decarboxylase△LdcEt3Alkaline stabilityCadaverine

Zhuang Li、Yaju Xue、Xiuling Ji、Yuhong Huang

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Beijing Key Laboratory of Ionic Liquids Clean Process,CAS Key Laboratory of Green Process and Engineering,State Key Laboratory of Multiphase Complex Systems,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,China

School of Chemical Engineering,University of Chinese Academy of Sciences,Beijing,100049,China

Innovation Academy for Green Manufacture,Chinese Academy of Sciences,Beijing,100190,China

Zhengzhou Institute of Emerging Industrial Technology,Zhengzhou,450000,China

Zhongke Langfang Institute of Process Engineering,Langfang 065001,China

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National Natural Science Foundation of ChinaBeijing Nova Program of Science and TechnologyInnovation Academy for Green Manufacture,CASNatural Science Foundation of BeijingHenan Key Research and Development ProjectHebei Provincial Natural Science FoundationCAS Pioneer Hundred Program

22078346Z201100006820141IAGM2020C192204097202102210046B2020103010

2023

10.1016/j.gce.2021.11.010

绿色化学工程(英文)
中国科学院过程工程研究所

绿色化学工程(英文)

CSCD
ISSN：
年,卷(期)：2023.4(2)
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