首页|Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass

Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass

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Glucose isomerase(GI)is an enzyme with high potential applications.Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential.Bacillus sp.,Paenarthrobacter sp.,Chrys-eobacterium sp.,Hymenobacter sp.,Mycobacterium sp.,and Stenotrophomonas sp.were isolated from soil samples.Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology.All isolates exhibited maximum GI activity at 40 ℃,pH 6-8 after 4 days of incubation.A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production.The same trend was observed in fermentation medium containing 1％xylose or 2％-2.5％wheat straw.This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry.

Cellulolytic bacteriaGlucose/xylose isomerase16S rRNABiomass conversion

Aristide Laurel Mokale Kognou、Chonlong Chio、Janak Raj Khatiwada、Sarita Shrestha、Xuantong Chen、Hongwei Li、Yuen Zhu、Zi-Hua Jiang、Chunbao(Charles)Xu、Wensheng Qin

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Department of Biology,Lakehead University,Thunder Bay,ON,P7B 5E1,Canada

Department of Chemical and Biochemical Engineering,Western University,London,ON,N6A 5B9,Canada

School of Environment and Resources,Shanxi University,Taiyuan,030006,China

Department of Chemistry,Lakehead University,Thunder Bay,ON,P7B 5E1,Canada

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Natural Science and Engineering Research Council of Canada(NSERC)Discovery Grant

RGPIN-2017-05366

2023

10.1016/j.gce.2022.05.003

绿色化学工程(英文)
中国科学院过程工程研究所

绿色化学工程(英文)

CSCD
ISSN：
年,卷(期)：2023.4(2)
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