首页|A benzothiazole-based ratiometric fluorescent probe with large Stokes shifts for quantitative profiling of sulfite in real samples and living cells

A benzothiazole-based ratiometric fluorescent probe with large Stokes shifts for quantitative profiling of sulfite in real samples and living cells

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Rapid,selective,and quantitative profiling of SO32-is a prerequisite for investigating its effect on different physiopathological processes.To address this challenge,our team prepared a ratiometric fluorescent probe HBTC-S,based on 4-(benzothiazol-2-yl)-3-hydroxybenzaldehyde for specific detection of SO32-under a single-wavelength excitation.The solution of probe HBTC-S itself exhibited orange fluorescence(λem=614 nm),and became green fluorescent(λem=471 nm)after the addition of SO32-.The vitro experiments suggested that the probe displayed high sensitivity,excellent selectivity,and a low detection limit(0.15 pM).Moreover,probe HBTC-S was successfully applied for qualitative and quantitative monitoring of SO32-in realistic samples and living HeLa cells in a ratiometric manner.

RatiometricFluorescent probeSulfite(SO32-)Single-wavelength excitationLiving imaging

Fengpei Qi、Xiuxiu Yue、Yitong Yang、Qin Liang、Ruiren Tang、Xiangzhi Song

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College of Chemistry&Chemical Engineering,Central South University,Changsha,410083,China

College of Materials & Chemical Engineering,Hunan City University,Yiyang,413000,China

国家自然科学基金Scientific Research Fund of Hunan Provincial Education Department of China

2217839520B104

2023

绿色化学工程(英文)
中国科学院过程工程研究所

绿色化学工程(英文)

CSCD
ISSN:
年,卷(期):2023.4(4)
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