首页|A benzothiazole-based ratiometric fluorescent probe with large Stokes shifts for quantitative profiling of sulfite in real samples and living cells
A benzothiazole-based ratiometric fluorescent probe with large Stokes shifts for quantitative profiling of sulfite in real samples and living cells
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维普
Rapid,selective,and quantitative profiling of SO32-is a prerequisite for investigating its effect on different physiopathological processes.To address this challenge,our team prepared a ratiometric fluorescent probe HBTC-S,based on 4-(benzothiazol-2-yl)-3-hydroxybenzaldehyde for specific detection of SO32-under a single-wavelength excitation.The solution of probe HBTC-S itself exhibited orange fluorescence(λem=614 nm),and became green fluorescent(λem=471 nm)after the addition of SO32-.The vitro experiments suggested that the probe displayed high sensitivity,excellent selectivity,and a low detection limit(0.15 pM).Moreover,probe HBTC-S was successfully applied for qualitative and quantitative monitoring of SO32-in realistic samples and living HeLa cells in a ratiometric manner.