Determination of 19 phenolic substances in citrus microcarpa by high performance liquid chromatography
In order to investigate the composition and content of phenols and citrulline in cit-rus microcarpa,a method was developed for the simultaneous determination of 19 phenols and citrulline citrus microcarpa using high performance liquid chromatography(HPLC),and the con-tents of phenols and citrulline were determined in different parts of Hainan citrus microcarpa(peel,pulp and juice).The results showed that the limits of detection(LOD)and the limits of quantification(LOQ)of the method were in the range of 0.052～2.344 pg/mL and 0.173～7.813 pg/mL,and the correlation coefficients in the linear range of the concentration were good(0.993～0.999).The retention time and RSD of peak area of each standard substance were in the range of 0.01％～0.32％and 0.84％～4.74％respectively,and the recoveries and RSD were in the range of 95.54％～101.64％and 0.11％～4.83％respectively,which showed the method has good precision and good qualitative and quantitative effects;the stability and reproducibility RSD were within 3.79％,indica-ting that the stability and reproducibility of the method are good.The main polyphenolic compounds in kumquat fruit were:3',5'-di-C-β-glucopyranosylphloretin,Hesperidin,Vicenin,Eriocitrin,and poncirin-2"-O-rhamnoside;a-mong them,3',5'-di-C-β-glucopyranosylphloretin was found in the fruit peel with a high content of 3 053.667 mg/100 g,which was significantly higher than other phenolic substances.The content and variety of phenolic compounds and citrinin in the pericarp were more than that in the pulp.
万方数据
维普
