Analysis of amino acid composition and antioxidant activity of fish swim bladder
One of the material bases of swim bladder is the composition of amino acids and a-mino acid components.The amino acid types and contents of swim bladder protein and its poly-peptides were determined and analyzed by an automatic amino acid analyzer.The results showed that both the swim bladder protein and its polypeptide contained 16 amino acids.The total amino acid content of fish bladder protein,fish bladder trypsin hydrolyzed peptide,fish bladder alkaline protease hydrolyzed peptide,and fish bladder pepsin hydrolyzed peptide was 92 043.85 ng/mg,79 402.03 ng/mg,71 978.95 ng/mg,335 053.03 ng/mg.Subsequently,fish bladder protein was used as the research object to conduct in vitro antioxidant experiments,release performance experiments,and in vivo experimental studies on the impact of oxidative damage on the sur-vival rate of nematodes.The experimental re-sults showed that fish swim bladder peptides has the ability to scavenge NO and hydroxyl radicals,and chelate ferrous and copper ions;The impact of oxidative damage on the survival rate of nematodes in vivo increases with the increase of protein concentration within a certain range,demonstrating good antioxidant ability.
fish swim bladderamino acidantioxidantactivity study
万方数据
维普
