This study successfully developed an immunochromatographic fluorescence quanti-tative detection card for lead in wheat.This detection card is based on the principle of competitive inhibition immunochromatography,using europium nanospheres as fluorescent probes to label an-ti Pb EDTA monoclonal antibodies,and constructing a quantitative immunochromatographic a-nalysis system.The research results show that the sensitivity of lead is 0.48 ng/mL,and the de-tection limit and quantification limit are 10.18 and 25.48 μg/kg,respectively;the determination recovery rate of certified reference materials is 86.6％-110％;the precision results show that the coefficient of variation(CV)is all below 15％.In addition,there was no significant difference between the detection results obtained by chromatography card and ICP-MS method,which ver-ified the accuracy and reliability of the method.
关键词
免疫层析荧光/重金属铅/小麦/定量检测
Key words
immunochromatographic fluorescence/heavy metal lead/wheat/quantitative de-tection
维普
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