The experiment used the uncertainty of measurement in determination of aflatoxin B,in sunflower seeds by HPLC-MS/MS is evaluated and studied the main sources of uncertain-ty.The results indicate when the content of aflatoxin B,in Sunflower seeds was 0.097 μg/kg,the expanded uncertainty was 0.016 μg/kg(k=2).The uncertainty mainly comes from volume un-certainty and HPLC-MS/MS instrument generated by the uncertainty.
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