Study on immobilized lipase of magnetic multi-carbon nanotubes and its catalytic synthesis of OPO
In this paper,magnetic multi-walled carbon nanotubes were used to immobilize the lipase of Thermoanaerobacter sparsus thermophilus(TLL)for the preparation of 1,3-dio-leoyl-2-palmitoylglycerol(OPO).The effects of TLL addition,reaction time,and reaction temperature on the immobilization conditions as well as the effects of immobilized TLL addition,reaction time,reaction temperature,and substrate molar ratio on the preparation of OPO were investigated.The results showed that the immobilised enzyme had better acid and alkali resist-ance and thermal stability.The optimal reaction conditions for the immobilisation were 6％en-zyme addition,5 h reaction time and 45 ℃;the optimal conditions for the synthesis of OPO were 6 h reaction time,50 ℃,8％enzyme addition and 5∶1 substrate molar ratio.
magnetic multi-walled carbon nanotubesimmobilizationlipase1,3-dioleoyl-2-palmitoylglycerol
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