In this experiment,collagen was extracted from pig skin by acid method.The effects of citric acid concentration,heating time,liquid-solid ratio and extraction temperature on collagen extraction rate were studied through single factor and response surface analysis experi-ment,and the response surface optimization was carried out with the extraction rate as the response value.The results showed that the collagen ex-traction rate of citric acid was higher than that of hydrochloric acid and acetic acid,and the influence on the extraction effect was extraction temperature＞citric acid concentration＞heating time＞liq-uid-solid ratio.The optimal process of collagen extraction was as follows:citric acid concentra-tion 0.5 mol/L,heating time 29 min,liquid-solid ratio 20.6 mL/g,extraction temperature 72 ℃,the extraction rate of collagen reached 82.7％under the process conditions.
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