Response surface methodology was used to optimize the acid extraction process of porcine skin collagen
In this experiment,collagen was extracted from pig skin by acid method.The effects of citric acid concentration,heating time,liquid-solid ratio and extraction temperature on collagen extraction rate were studied through single factor and response surface analysis experi-ment,and the response surface optimization was carried out with the extraction rate as the response value.The results showed that the collagen ex-traction rate of citric acid was higher than that of hydrochloric acid and acetic acid,and the influence on the extraction effect was extraction temperature＞citric acid concentration＞heating time＞liq-uid-solid ratio.The optimal process of collagen extraction was as follows:citric acid concentra-tion 0.5 mol/L,heating time 29 min,liquid-solid ratio 20.6 mL/g,extraction temperature 72 ℃,the extraction rate of collagen reached 82.7％under the process conditions.
NETL
NSTL
万方数据
