Study of pretreatment conditions in preparing wheat bran oligosaccharides
The effect of content of high temperature resistant alpha amylase,enzymolysis time on removing starch from wheat bran was reseached;and the effect of acid type,cooking temperature on the quantity of soluble xylan was also studied. The results showed that high temperature resistant alpha amylase 0.2%and enzymolysis time 15 min were the optimal conditions to remove starch;the optimal process of cooking with acidic conditions were as follows:destarch wheat bran added 0.012 mol/L HCL solution by the ratio 1∶6,temperature 120℃,cooking time 30 min. HPLC analysis showed:the content of xylooligesaccharides in wheat bran oligosaccharides was about 52.5%;main components were glucose,arabinose,xylose,and their content is1∶3∶0.75.
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万方数据
维普
