马齿苋黄酮对油脂的抗氧化作用研究
Antioxidant effects of flavonoid of Portulaca oleracea L. on oils
艾洪超 1张文泉 1唐红枫2
作者信息
- 1. 武汉东湖学院生命科学与化学学院，湖北武汉430212
- 2. 武汉东湖学院生命科学与化学学院，湖北武汉430212; 武汉东湖学院应用生物技术重点实验室，湖北武汉430212
- 折叠
摘要
研究了马齿苋黄酮提取优化工艺和其对油脂的抗氧化作用。利用Gentle MACS Dissociator匀浆技术，结合单因素和正交试验法对马齿苋黄酮进行醇提，探究影响马齿苋黄酮提取率的主要因素，随后利用提取的马齿苋黄酮进行油脂的抗氧化研究。结果表明：马齿苋经Gentle MACS Dissociator匀浆，最佳提取条件为在65℃下加入35倍75%乙醇提取75 min，黄酮得率为7.51%。马齿苋黄酮能显著地减缓油脂的过氧化作用，其抗氧化活性在豆油中明显优于0.02%维生素E，并且0.02%维生素E对马齿苋黄酮的抗氧化作用表现出一定的增效协同作用。
Abstract
To study the main factors effecting extractionof the purslane flavonoid,the Gentle MACS Dissociator homogenate technology was used to gain theoptimal extractionconditionsof purslane flavonoid by single factor method andorthogonal experiment method.Then the purslane flavonoid was investigated about antioxidant activitiesonoils.The results showed that theoptimal extractionconditions of purslane flavonoid were determined as follows:temperatureof extraction at 65℃,solvent–solid ratio 30∶1,temperature time 75 min,the extraction yieldof total flavonoids was 7.51%. Antioxidative effectof purslane flavonoidonoils was studied. The results showed that purslane flavonoidcould reduce the reactionof lipid peroxidationofoil significantly.The antioxidant activityon soybeanoil was better than 0.02% vitamin E. What’s more,antioxidative activityof purslane flavonoid mixed with vitamin E might exhibit synergistic effect.
关键词
马齿苋/黄酮/正交试验/抗氧化性Key words
Portulacaoleracea L./flavonoid/orthogonal experiment/antioxidation引用本文复制引用
基金项目
武汉东湖学院2014年度大学生重点科研项目()
出版年
2015
NETL
NSTL
维普
万方数据