The purification process of yam peel polyphenol using macroporous resin was studied,and the scavenging ability of purified polyphenol to DPPH·,ABTS+·,·O2-and·OH was evaluated.The results showed that X-5 was the best resin for purifying yam peel polyphenol.The optimal purification process was pH 6.0 of sample solution,mass concentration 0.6 mg/mL of sample solution,volume frac-tion of ethanol(elution)80％,sample flow rate 2 mL/min,elution flow rate 2 mL/min.The adsorption isotherm was consistent with Langmuir monolayer adsorption.After purification under these conditions,the purity of polyphenols extract was increased from 34.09％to 80.22％.The antioxidant activity test showed that the purified yam peel polyphenols had significantly higher scavenging capacity for DPPH·,ABTS+·,·OH and·O2-than the crude extract,and had strong antioxidant activity,which was a nat-ural antioxidant with potential development.
NETL
NSTL
万方数据
