粮食与油脂2025,Vol.38Issue(1) :82-86.

大豆低聚肽双蛋白酸奶的研制及其活性益生菌数量评价

Development of soybean oligopeptide dual-protein yogurt and evaluation of its active probiotics count

薛红梅 陆少兰 刘玉美 刘晓松 张雪苍
粮食与油脂2025,Vol.38Issue(1) :82-86.

大豆低聚肽双蛋白酸奶的研制及其活性益生菌数量评价

Development of soybean oligopeptide dual-protein yogurt and evaluation of its active probiotics count

薛红梅 1陆少兰 2刘玉美 1刘晓松 1张雪苍1
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作者信息

  • 1. 陕西省粮油科学研究院,陕西西安 710082
  • 2. 陕西省粮油科技开发公司,陕西西安 710082
  • 折叠

摘要

使用含双歧杆菌属、乳杆菌属、嗜热链球菌属等12种菌型的发酵剂,在牛奶中添加大豆分离蛋白、大豆低聚肽,通过巴氏杀菌后发酵制备双蛋白酸奶.以感官评分为指标,基于单因素试验结果,运用响应面法优化双蛋白酸奶配方,并与市售冷藏固体发酵酸奶对比分析其活性益生菌数量.结果表明:最佳配方为以全脂牛奶质量为基准,大豆低聚肽添加量0.7%、大豆分离蛋白添加量1.4%、蔗糖添加量8.0%.在此条件下制作的双蛋白酸奶口感最佳,风味独特;与市售冷藏固体发酵酸奶相比,大豆低聚肽双蛋白酸奶中的双歧杆菌属、乳杆菌属、嗜热链球菌属含量均显著增加.

Abstract

Using a fermentation agent containing 12 strains of bacteria including Bifidobacterium,Lacto-bacillus and Streptococcus thermophilus,the dual-protein yogurt was prepared by pasteurization and fer-mentation after adding soybean protein isolate and soybean oligopeptide into milk.Taking sensory evalua-tion as indicator,response surface methodology was used to optimize the dual-protein yogurt formula based on the results of single factor experiments,and the active probiotics count was compared and ana-lyzed with commercially available refrigerated solid-state fermented yogurt.The results showed that the optimal formula was based on the mass of whole milk,addition of soybean oligopeptide 0.7%,addition of soybean protein isolate 1.4%,and addition of sucrose 8.0%.Under these conditions,the dual-pro-tein yogurt obtained had the best taste and unique flavor.Compared with commercially available refriger-ated solid-state fermented yogurt,the content of Bifidobacterium,Lactobacillus,and Streptococcus ther-mophilus in soybean oligopeptide dual-protein yogurt had significantly increased.

关键词

大豆低聚肽/双蛋白/酸奶/益生菌

Key words

soybean oligopeptide/dual-protein/yogurt/probiotic

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出版年

2025
粮食与油脂
上海市粮食科学研究所

粮食与油脂

北大核心
影响因子:0.622
ISSN:1008-9578
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