Optimization of the extraction process of crude polysaccharide from Auricularia polytricha by response surface methodology and evaluation of its antitumor activity
The crude polysaccharide from Auricularia polytricha was extracted by hot water extraction.The comprehensive score was calculated based on the yield of crude polysaccharide,total sugar content,and biological activity evaluation as weighting indicators.Single factor experiments and response surface experiment were used to optimize the extraction process of the crude polysaccharide from Auricularia polytricha and its antitumor activity was evaluated.The results showed that the optimal extraction parame-ters were crushed particle size 0.125 mm,extraaction solution pH 9,extraction once,extraction temper-ature 60 ℃,solid-to-liquid ratio 1∶100(g/mL),and extraction time 60 min.Under these conditions,the comprehensive score of the crude polysaccharide from Auricularia polytricha was 64％.The antitu-mor activity assay result demonstrated that the crude polysaccharide from Auricularia polytricha signifi-cantly reduced the tumor mass and the number of lung metastatic nodules in 4T1 breast cancer mice,in-dicating it had a good role in antitumor activity.
crude polysaccharide from Auricularia polytrichaextraction processresponse surface methodologybiological activityantitumor activity
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