The effect and mechanism of Gastrodia elata probiotics compound on sleep improvement in mice were explored.A total of 70 Balb/c mice were randomly divided into blank control group,positive drug group,low dose of Gastrodia elata ethanol extract group,high dose of Gastrodia elata ethanol extract group,probiotics group,low dose Gastrodia elata combined with probiotics group and high dose Gastro-dia elata combined with probiotics group.The sleep-improving effect was evaluated by the mice voluntary activity test,the upper and subthreshold dose of pentobarbital sodium hypnotic test,and the levels of γ-aminobutyric acid(GABA)content and dopamine(DA)content in mice brain tissue,and norepineph-rine(NA)in serum.The results showed that the high dose of Gastrodia elata combined with probiotics could significantly inhibit the autonomic activity of mice,increase the sleep onset rate of mice at the sub-threshold dose,shorten the sleep onset latency and prolong the sleep duration of mice at the suprathresh-old dose,increase the content of GABA in mice brain tissue,and reduce the content of DA in mice brain tissue and NA in serum.In conclusion,the combination of high dose Gastrodia elata ethanol extract and probiotics could improve the sleep quality of mice.
Gastrodia elataprobioticinsomniasedative and hypnoticneurotransmitter
维普
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