摘要
对淡竹叶黄酮(LGF)组分进行分析,并探讨其对血管紧张素(Ang Ⅱ)诱导心肌细胞损伤的改善作用.结果表明:LGF主要由异荭草苷、绿原酸、苜蓿素、荭草苷、异牡荆苷、芸香糖苷、木犀草苷、槲皮素、牡荆苷组成,摩尔比为 3.36∶4.22∶1.15∶20.59∶17.99∶13.19∶2.54∶1∶7.97.与 M-Ang Ⅱ相比,高(5 mg/mL)、中(2.5 mg/mL)、低(1 mg/mL)剂量LGF组心肌细胞表面积均显著减小,心肌细胞丙二醛(MDA)含量分别显著降低了 77.54％、57.85％、48.31％,抗氧化酶活性(SOD、CAT、GSH-Px)均显著升高,且均与剂量呈依赖关系.心肌细胞表达分析结果显示,与M-Ang Ⅱ相比,高、中、低剂量LGF组心肌细胞内促凋亡因子(Bax、Caspase-3、p53)mRNA相对表达水平均显著或极显著降低,抗凋亡因子Bcl-2 mRNA相对表达水平极显著升高.总体表明LGF能够改善Ang Ⅱ诱导的心肌细胞损伤,其作用机制可能与降低细胞氧化应激水平和抑制细胞凋亡有关.
Abstract
The components of Lophatherum gracile Brongn.flavonoids(LGF)were analyzed,and the improvement effects of LGF on cardiomyocyte injury induced by angiotensin(Ang Ⅱ)were investigated.The results showed that LGF was mainly composed of isoorientin,chlorogenic acid,tricin,orientin,iso-vitexin,rutinoside,cynaroside,quercetin and vitexin,with a molar ratio of 3.36∶4.22∶1.15∶20.59∶17.99∶13.19∶2.54∶1∶7.97.Compared with M-Ang Ⅱ,the surface area of cardiomyocyte in the high(5 mg/mL),medium(2.5 mg/mL),and low(1 mg/mL)dose LGF groups were significantly reduced.The contents of malondialdehyde(MDA)in cardiomyocytes were significantly reduced by 77.54％,57.85％,and 48.31％,respectively.The antioxidant enzyme activities(SOD,CAT,GSH Px)were significantly increased,showing a dose-dependent relationship.The analysis of cardiomyocyte expression showed that compared with M-Ang Ⅱ,the mRNA relative expression levels of pro-apoptotic factors(Bax,Caspase-3,p53)in cardiomyocytes of high,medium,and low dose LGF groups were significantly or ex-tremely significantly reduced,while the mRNA relative expression level of anti-apoptotic factor Bcl-2 was extremely significantly increased.Overall,it showed that LGF could improve Ang Ⅱ-induced cardiomyo-cyte injury,and its mechanism of action may be related to reducing cellular oxidative stress levels and in-hibiting cell apoptosis.
维普
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