In order to study the effect of sucrose on papain activity and its molecular mechanism,papain was treated with 5 different concentrations(0,0.25,0.50,0.75,1.00 mol/L)of sucrose solution,the enzyme activity was determined,and molecular simulation,molecular docking and fluorescence analysis were performed under 5 concentrations.The root mean square error(RMSD)and secondary structure of papain were analyzed.The results showed that sucrose could promote the activity of papain enzyme,and the promotion effect was strongest when the concentration was 0.25 mol/L,but the relative activity of the enzyme decreased with the increase of sucrose concentration.The molecular simulation results showed that sucrose could reduce the fluctuation of RMSD and make the protease structure more stable.When the concentration was 0.25 mol/L,the structure of the active center of papain became more stable and closely to the substrate,which could effectively promote the catalytic reaction.Therefore,the addition of sucrose solution caused changes in the structure of papain active center and promoted the activity of papa-in enzyme.
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