高效液相色谱-串联质谱法测定鸡蛋中18种拟除虫菊酯类农药残留

Determination of 18 pyrethroid pesticide residues in eggs by high performance liquid chromatography tandem mass spectrometry

唐俗 ¹卓程梆 ¹朱雨田 ¹徐述龙 ¹杜业刚 ¹罗淙岚²

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作者信息

1. 深圳市计量质量检测研究院,广东深圳 518131
2. 华南农业大学食品学院,广东广州 510642
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摘要

基于高效液相色谱-串联质谱法(LC-MS/MS)建立同时测定鸡蛋中18种拟除虫菊酯类农药残留的方法.样品经10 mL乙腈提取,弗罗里硅土固相萃取柱净化,以5 mmol/L乙酸铵溶液(A)-甲醇(B)作为流动相进行梯度洗脱,在离子源温度450 ℃时,采用多反应监测模式(MRM)进行定性定量分析.结果表明:18种拟除虫菊酯类农药在质量浓度5.00～200.00 μg/L范围内,线性关系良好(r＞0.995),检出限为5.00μg/kg,在20.0～100 μg/kg加标水平下,回收率为79.2％～107％,相对标准偏差为 0.707％～8.91％(n=3).

Abstract

A method for simultaneous determination of 18 pyrethroid pesticide residues in eggs based on liquid chromatography tandem mass spectrometry(LC-MS/MS)was established.The sample was extrac-ted with 10 mL of acetonitrile and purified by a Florisil solid phase extraction column.A gradient elution was performed using 5 mmol/L ammonium acetate solution(A)-methanol(B)as the mobile phase.Qualitative and quantitative analysis was performed using multiple reaction monitoring mode(MRM)at ion source temperature of 450 ℃.The results showed that 18 pyrethroid pesticides had good linear rela-tionship(r＞0.995)in the mass concentration range of 5.00-200.00 µg/L.The limit of detection was 5.00 μg/kg.At the spiked level of 20.0-100 μg/kg,the recovery rate was 79.2％-107％,and the relative standard deviation was 0.707％-8.91％(n=3).

关键词

拟除虫菊酯/鸡蛋/高效液相色谱-串联质谱法(LC-MS/MS)

Key words

pyrethroid/egg/high performance liquid chromatography tandem mass spectrometry(LC-MS/MS)

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出版年

2025

粮食与油脂

上海市粮食科学研究所

粮食与油脂

北大核心

影响因子：0.622

ISSN：1008-9578

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