Tissue Culture of Medicinal and Edible Plant Kadsura coccinea
In view of the fact that the wild resources of Kadsura coccinea,a medicine and food homolo-gous plant,are decreasing day by day,and it is difficult to meet the market demand,the preparation of culture medium and the cultivation conditions of tissue culture bottle seedlings in tissue culture rapid propagation system were studied,in order to establish a tissue culture rapid propagation system of K.coccinea and lay a foundation for large-scale seedling propagation.The results showed that MS+2.0 mg/L 6-BA+0.2 mg/L NAA was the primary culture medium,B5+2.0 mg/L 6-BA+0.2 mg/L NAA was the proliferation culture medium,and 1/2 MS+0.1 mg/L 6-BA+1.5 mg/L NAA+0.3%activated carbon was the rooting culture medium.The rooting rate could reach 100%,and the main root of the seedlings was thick and strong in growth when cultivated in dark and then under light.The best hardening time of tissue culture seedlings was 15 days,the best transplanting medium was pond mud∶bagasse∶chicken manure∶superphosphate=3.9∶4∶2∶0.1,the best shading mode was half shading,and the best cultivation period was 60 days.
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