The indoor toxicity of five fungicides against strawberry anthracnose pathogen was measured using the mycelial growth rate method.Two fungicides with excellent effect were compoun-ded to screen the synergistic ratios,and the control effect with the maximum co-toxicity coefficient was verified in greenhouse.The results showed that both difenoconazole and pyraclostrobin exhibited good inhibitory effects on strawberry anthracnose,and the EC50 values were 0.80 mg/L and 6.33 mg/L,respectively.The synergistic coefficients of difenoconazole and pyraclostrobin were 0.70 and 1.51,respectively.The co-toxicity coefficient of 2:1 ratio was the best.In greenhouse,diluted 1 500~2 000 times with a 30%difenoconazole·pyraclostrobin suspension(pyraclostrobin and difenoconazole 2:1)and treated with root dipping during transplantation had a good control effect on strawberry anthracnose and had no negative impact on strawberry growth.