Whole genome cloning and preliminary identification of CLBV strain in Prunus
In this study,small RNA high-throughput sequencing was used to identify a virus with high homology to the citrus leaf blister virus(CLBV)of the genus beta-linear virus in sweet cherry.The whole genome of the virus was cloned,and it was found that the virus had 80%homolo-gy with CLBV at nucleotide level;the sequence homology of the coat protein gene of this virus was compared with that of CLBV.The homology was 79%at the nucleotide level and 88%at the amino acid level.According to the classification criteria,this virus was considered a new strain of CLBV,and named Citrus Leaf Blotch virus Strain Prunus.