Chromosome Karyotype and G-band Analysis of Bovine Small Intestinal Epithelial Cell Line
In order to identify the genetic stability in biology of bovine small intestinal epithelial cell line(BIECs-21),BIECs-21 cells were cultured in vitro by using adherent culture,and chromosome and G-band samples were prepared to analyze the chromosome karyotype and G-band of BIECs-21 cells.The results show that the number of chromosomes in BIECs-21 cells is 2n=60 including 29 pairs of autosomes and 1 pair of sex chromosomes(X and Y chromosomes).Among them,29 pairs of autosomes are all terminal centromere chromosomes,with X chromosome being the larger centromere chromosome and Y chromosome being the smaller centromere chromosome in the sex chromosome.There is no significant difference in the number and position of G-bands pattern on the autosomes of BIECs-21 cells,except for X and Y sex chromosome.Compared with the normal chromosome karyotype of Holstein cattle,there is no significant change in the number and morphological structure of chromosomes in BIECs-21 cells,indicating that the genetic characteristics of the cell line are stable,and it can be used for the reaearch on the molecular mechanisms of bovine intestinal related diseases.
国家科技期刊平台
NSTL
万方数据
