Optimization of SRAP marker reaction system and genetic diversity in Eucalyptus
The orthogonal design was used to optimize a SRAP-PCR system with 4 factors (Mg2+ , dNTP, primer and Taq polymerase) at 3 levels. The effect of the concentration of template DNA on PCR amplification was compared. The optimized SRAP-PCR system in Eucalyptus was l0ng template DNA, 2. 5 mmol/L Mg2+, 0. 2mmol/L dNTP, 0. 4μmol/L primer, and l.SU Taq polymerase in a total of 10μL reaction solution. The selected twelve primer pairs out of forty were polymorphic by the optimized SRAP-PCR. Twelve primer pairs produced 109 bands among 11 accessions, showing some genetic diversity in Eucalyptus. The result indicated that SRAP molecular marker could be widely used in molecular research in Eucalyptus.
国家科技期刊平台
NSTL
万方数据
维普
