Comparison of two universal liquid chromatography detectors for determination of carbohydrates in forest biomass
Forest biomass mainly consists of cellulose and hemicellulose.The ratio of these components is important in assessing the usefulness of forest biomass resources and optimizing its processing technology in practical applications.High-performance liquid chromatography(HPLC)equipped with a refractive index detector(RID)was commonly used for compound analysis in the previous studies.A comparative study of the RID and evaporative light scattering detector(ELSD)methods for analyzing carbohydrate components has not been reported in the biomass research field.The objective of this study was to evaluate the sugar properties for the usefulness of Pinus spp and Populus tomentosa through the two universal detectors,namely RID and ELSD.To achieve this,the detection conditions were optimized,and the applicability of both detection methods was compared.Additionally,the properties of six types of sugar,including cellobiose,glucose,xylose,galactose,arabinose,and mannose,were evaluated.This comprehensive evaluation helped for a detailed assessment of the high-value utilization of forest biomass resources.Linear regression equations were used to evaluate the two detection methods,providing a comprehensive understanding of their relationship.These included stability,spiked recovery rate,linear range,minimum detection limit,and reproducibility.By using these equations,the performances of the two methods were thoroughly evaluated and the ranges within their operations were optimally determined.This study found that the RID and ELSD detectors of linear regression equations had a correlation coefficient greater than 0.999 60,with a minimum detection limit of 0.1-0.2μg.Additionally,the sensitivity of both detectors was similar,and their recoveries and sample stability were the same.After the thorough analysis and comparison,it was revealed that the RID detector had good linear correlation,a wide linear range,high precision,and good reproducibility in quantitatively analyzing high carbohydrate content from 9.0 to 900.0 μg.However,it was susceptible to changes in ambient temperature and had a long baseline equilibrium time.On the other hand,the ELSD detector had a narrow linear range and high precision in the quantitative analysis of low-content sugars ranging from 0.5 to 5.0 μg.The baseline stability was relatively fast and suitable for gradient elution methods.This study discovered that the levels of cellulose and hemicellulose in two species of Pinusspp and Populus tomentosa Carrière matched the expected values reported in scientific literature.This suggested that the ELSD detection method can be a promising new method for analyzing the sugars present in biomass from trees.
forest biomasssugarsrefractive index detectorevaporative light scattering detector
万方数据
维普
